Insertion junction: CLIP2.072252_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.072252
Chromosome:chromosome 2
Location:7197732
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g390763 RAB2,FAP354 Rab-like GTP-Binding Flagellar Associated Protein 354; (1 of 1) K07877 - Ras-related protein Rab-2A (RAB2A) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACGATCCTCGCGAAAGAAACTCGCTTCTTCTTTCCACTTCTTCCACCCC
Internal bar code:CGTAGACAATTTTGGCGTCTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:4674
LEAP-Seq percent confirming:87.1429
LEAP-Seq n confirming:61
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:70

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTCAGCGCACGTAATTCG
Suggested primer 2:TGGCATAGGAGGTCTCTGCT