Insertion junction: CLIP2.072263_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.072263
Chromosome:chromosome 11
Location:140677
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467538 GOX10,GOX8 (1 of 20) PTHR32208//PTHR32208:SF21 - FAMILY NOT NAMED // F10A5.18; Glyoxal oxidase 8 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGGGGCTCTCAAGCGCTGGTGCGAATGATTTCATGTTTAGAAGGCTGCT
Internal bar code:AACACGCAACGAGAGACAGATG

Confirmation - LEAP-Seq

LEAP-Seq distance:307
LEAP-Seq percent confirming:4.54545
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:21
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:ACTCATGGATCTGTCCCCGA
Suggested primer 2:GCAGTGACTCCGCAACTTTG