Insertion junction: CLIP2.072268_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.072268
Chromosome:chromosome 10
Location:496200
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g421050 (1 of 9) 2.7.10.1 - Receptor protein-tyrosine kinase / Receptor protein tyrosine kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGAACACCAGCAGTGACGCCGCCGGCAGCCACAACGACTCACAGTCCGC
Internal bar code:TTGTTTAGTGTAGTTTTGCTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:4035
LEAP-Seq percent confirming:94.5946
LEAP-Seq n confirming:35
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:ATCACCACACGCCTGCTTTA
Suggested primer 2:ATTGCCAAGGCCGTCAAAAC