Insertion junction: CLIP2.072279_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.072279
Chromosome:chromosome 1
Location:2051151
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g011300 SCLP50,CPY Serine carboxypeptidase; (1 of 3) K09645 - vitellogenic carboxypeptidase-like protein [EC:3.4.16.-] (CPVL) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGATAACGGTAGAATGGGTTGAGGCGAAGTGAGCGGGATTGCCAAATGGG
Internal bar code:GCTAGGATAACACGTATAATGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1285
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:47
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:47

Suggested primers for confirmation by PCR

Suggested primer 1:TGTCCAGCTATCCCGTCTCA
Suggested primer 2:TGGGCTGCTATCTACACCCT