| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.072291 |
| Chromosome: | chromosome 7 |
| Location: | 6117950 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g355650 | AMT1F,AMT6 | Ammonium transporter; (1 of 8) K03320 - ammonium transporter, Amt family (amt, AMT, MEP) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTGCTGTGAGCACACCGCCACTACCTGCCGCCTCAGCTGGAGCCCGGT |
| Internal bar code: | TATAGGAAATTCAGGTCGATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 95 |
| LEAP-Seq percent confirming: | 12.5 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCACTTCGTCTGCAGTCT |
| Suggested primer 2: | CGTTCACTCCGTTCTCCACA |