| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.072292 |
| Chromosome: | chromosome 7 |
| Location: | 2977322 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g333100 | 3'UTR | ||
| Cre07.g333150 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGACTCCTGGAGATTGGATGGCCTCACAGGTGCATCAGCTTCCAGCCAGA |
| Internal bar code: | GTGGCGATCGGTGCAGTGTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2089 |
| LEAP-Seq percent confirming: | 16.6667 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGTACATATCACGCGTCC |
| Suggested primer 2: | CTGCCTGTGTTGCTTGTTCC |