Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.072312 |
Chromosome: | chromosome 3 |
Location: | 9252639 |
Confidence (%): | 40 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g800424 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTTGGGCTTGGGTTTGGATCCAGATCCAGGAGATTCGGATCCAGATCC |
Internal bar code: | AACAAGGGCAGGCATTCGTAAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 114 |
LEAP-Seq percent confirming: | 38.2353 |
LEAP-Seq n confirming: | 13 |
LEAP-Seq n nonconfirming: | 21 |
LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CACAGACCACGGGTTCCTTT |
Suggested primer 2: | CTCCAATCCTCCCCAAACCC |