| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.072321 |
| Chromosome: | chromosome 12 |
| Location: | 7801101 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g553950 | (1 of 33) IPR013830 - SGNH hydrolase-type esterase domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGCGGCGCCCATGCCGCTGTAGCTCTGTAGGTAGGCGACCTGCGTGAG |
| Internal bar code: | GGTGTGGGACAGCTCTTGGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 248 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAACTTGACCACTCCGAGC |
| Suggested primer 2: | GTGCACGTGAAACTCACCAC |