| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.072332 |
| Chromosome: | chromosome 6 |
| Location: | 5361420 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g285100 | 3'UTR | ||
| Cre06.g285150 | APx-R,APX2 | Ascorbate peroxidase-related; (1 of 3) 1.11.1.11 - L-ascorbate peroxidase / Ascorbic acid peroxidase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGACCCCCTGCATCCTCTGATGCATCCTCTGAACAGCGGGCAGCCCTG |
| Internal bar code: | TAGCTCTAATATGTTCTAGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1145 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTACCTCGCTACGCTTCC |
| Suggested primer 2: | GTATCACGGTAGACTGGCGG |