| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.072341 |
| Chromosome: | chromosome 17 |
| Location: | 5818270 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g741350 | KIN7-4,KIN7D | Kinesin motor protein; (1 of 4) K11498 - centromeric protein E (CENPE) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAACCGGGTGCTAGCAAATCACAAAGCCAGAAACCGCTGTAACGTTTTCA |
| Internal bar code: | GAGTAGCTGCGCCAATAATCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1834 |
| LEAP-Seq percent confirming: | 96.9697 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTGACTCCGTTGACCAGA |
| Suggested primer 2: | CGCCGGGCTTTACTATAGCA |