| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.072343 |
| Chromosome: | chromosome 12 |
| Location: | 9560043 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g539900 | (1 of 1) K00166 - 2-oxoisovalerate dehydrogenase E1 component alpha subunit (BCKDHA, bkdA1) | 5'UTR | |
| Cre12.g540051 | TMEM70 | (1 of 1) PTHR13281:SF0 - TRANSMEMBRANE PROTEIN 70, MITOCHONDRIAL; Transmembrane protein 70, mitochondrial | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCACTGCTTTCTTGTGTCAATGACGTTACTTAATAGCTAATACACACA |
| Internal bar code: | GAGGTGCCTACGTAAAGTTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 955 |
| LEAP-Seq percent confirming: | 16.6667 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTGCGAATGTCTGGCTGG |
| Suggested primer 2: | ATGGTGTCCATGGTCTGCAG |