| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.072350 |
| Chromosome: | chromosome 1 |
| Location: | 2912624 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g800057 | (1 of 1) K00587 - protein-S-isoprenylcysteine O-methyltransferase (ICMT, STE14) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATAGTTCATCTTTTGGTTGCGTATCTTCGTACTCTGGGCCCGGCAGGCT |
| Internal bar code: | ACCAAGCGTCCTATTACGCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3932 |
| LEAP-Seq percent confirming: | 59.0909 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGCCTCCACGCTGTACTC |
| Suggested primer 2: | GCATGGGGGTTATCCTACCG |