Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | + |
Strain: | CLIP2.072351 |
Chromosome: | chromosome 6 |
Location: | 4703597 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g279050 | (1 of 7) PTHR11584:SF381 - PROTEIN PQN-80, ISOFORM B | 5'UTR_intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGACTAGGTCGTTTTGTGGGGGCTGAGCCCTAAGGCCCCGGCCCTGGTT |
Internal bar code: | AGGGCCAACATACTGGCATGGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1450 |
LEAP-Seq percent confirming: | 33.3333 |
LEAP-Seq n confirming: | 6 |
LEAP-Seq n nonconfirming: | 12 |
LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GACAGGCACGTTTTGAGCTG |
Suggested primer 2: | GGTTGCCCTACCTCTCACAC |