Insertion cassette: | CIB2 |
Side of cassette: | 5' truncated? |
Strand: | + |
Strain: | CLIP2.072357 |
Chromosome: | chromosome 5 |
Location: | 1072984 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre05.g247850 | (1 of 3) 3.6.1.22//3.6.1.55 - NAD(+) diphosphatase / NADP pyrophosphatase // 8-oxo-dGTP diphosphatase / 8-oxo-dGTPase | 5'UTR | |
Cre05.g247851 | CEP2 | Cysteine endopeptidase; (1 of 2) 3.4.22.14 - Actinidain / Actinidin | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTATGCAGAGTACAAGGAATAACAAACGCGATAGAAACGTTTGGCCAT |
Internal bar code: | CGGCAGTACTAGGTTGTGAGAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 891 |
LEAP-Seq percent confirming: | 33.3333 |
LEAP-Seq n confirming: | 3 |
LEAP-Seq n nonconfirming: | 6 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GATCGCGTGTTTCTGATGGC |
Suggested primer 2: | ACTATCATATGGGTGCGCGG |