Insertion junction: CLIP2.072357_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.072357
Chromosome:chromosome 5
Location:1072984
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g247850 (1 of 3) 3.6.1.22//3.6.1.55 - NAD(+) diphosphatase / NADP pyrophosphatase // 8-oxo-dGTP diphosphatase / 8-oxo-dGTPase 5'UTR
Cre05.g247851 CEP2 Cysteine endopeptidase; (1 of 2) 3.4.22.14 - Actinidain / Actinidin 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCTATGCAGAGTACAAGGAATAACAAACGCGATAGAAACGTTTGGCCAT
Internal bar code:CGGCAGTACTAGGTTGTGAGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:891
LEAP-Seq percent confirming:33.3333
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GATCGCGTGTTTCTGATGGC
Suggested primer 2:ACTATCATATGGGTGCGCGG