| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.072364 |
| Chromosome: | chromosome 5 |
| Location: | 2325249 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g238280 | FAP205 | Flagellar Associated Protein 205 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAAGTAGTATTTTGTTGTGTTTGATTGCGGGGGCGGAGTTGGGGGGCGG |
| Internal bar code: | ATGTTCCCCCTCAGTGTCCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 137 |
| LEAP-Seq percent confirming: | 40.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAACATGGCCGCAACAAA |
| Suggested primer 2: | CAACCCAACCCTACCCATCC |