Insertion junction: CLIP2.072369_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.072369
Chromosome:chromosome 8
Location:3826386
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g381483 SDR15 (1 of 239) IPR016024 - Armadillo-type fold; Short-chain dehydrogenase/reductase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTATAACTTGGTCCCGGCGATCGTGTCCGACGTCGACGGGTGACTTGGCT
Internal bar code:TTCCAACTTAATGAATCGTAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:722
LEAP-Seq percent confirming:17.2414
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:24
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:CGAGCTATCTCCTGGCACTG
Suggested primer 2:TGGATTTACGCGACTCGAGG