| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.072377 |
| Chromosome: | chromosome 1 |
| Location: | 7032972 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g050316 | (1 of 4) PTHR11229:SF5 - 50S RIBOSOMAL PROTEIN L3-1, CHLOROPLASTIC | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGCGAGGACATCCAGCGGCTCAAGGACCAGCACAACGAGCTGACGCAG |
| Internal bar code: | GAGGGATTCACGTTTCGATCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2870 |
| LEAP-Seq percent confirming: | 46.6667 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 105 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCTGAGTGGGAGAGCAAC |
| Suggested primer 2: | GGGATTTTGGAAGGAGGGGG |