| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.072385 |
| Chromosome: | chromosome 6 |
| Location: | 1643153 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g261950 | ANK11 | (1 of 29) PF13637 - Ankyrin repeats (many copies) (Ank_4); Predicted protein with ankyrin repeats | 5'UTR |
| Cre06.g262000 | SNR5 | SNARE-associated Golgi protein; (1 of 6) PF09335 - SNARE associated Golgi protein (SNARE_assoc) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTGATGGAAAAGGTGCTTTGGACAACGCACGAGCCGCGCCAGGCAGGG |
| Internal bar code: | ATGGGTGGGCACGGTTCGTTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1143 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 52 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATAGCTCCCCTGTACGTGC |
| Suggested primer 2: | CCATGTCCTGTCCCAATGCT |