| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.072396 |
| Chromosome: | chromosome 6 |
| Location: | 7499396 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g300933 | (1 of 2) PF15919 - HicB_like antitoxin of bacterial toxin-antitoxin system (HicB_lk_antitox) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTTTAAGTTTCTGTGCCTCGATCCTTTCGCCCCACAGCGCGCCGCCGC |
| Internal bar code: | GTCTTCGGGTAGGGTTCTCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6035 |
| LEAP-Seq percent confirming: | 97.8022 |
| LEAP-Seq n confirming: | 89 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 91 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGCTTCGTGTGAGTGTGA |
| Suggested primer 2: | GACGCTGAGGAGATACTGGC |