Insertion junction: CLIP2.072398_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.072398
Chromosome:chromosome 13
Location:4870582
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g801581 (1 of 17) 3.1.4.53 - 3',5'-cyclic-AMP phosphodiesterase / cAMP-specific phosphodiesterase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTGATTGAGAGGGGTGTTCATCTTTTATAGTCCGGTGTGGAGGGACGC
Internal bar code:CGGTCTTGTAGGCTATGAGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:289
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ACATAGCGCGGGTCATAGTG
Suggested primer 2:AATGGGGTACTGAGCGAACG