| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.072402 |
| Chromosome: | chromosome 10 |
| Location: | 4164966 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g449000 | VPT1,LZY1 | LZY1B homolog | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAATGTCCTTGCAACACTTGAAGTTATCTAGGCAGACGACGCCACAACCC |
| Internal bar code: | AGGTGTCTTTCACAACTTCAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1335 |
| LEAP-Seq percent confirming: | 98.2759 |
| LEAP-Seq n confirming: | 57 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCGTGACTGGAAGCTCAA |
| Suggested primer 2: | GAGCACAACATAGCGCCATG |