| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.072418 |
| Chromosome: | chromosome 3 |
| Location: | 4784416 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g177900 | TOB38,SAM38,TOM37,MTX1 | Metaxin 1; (1 of 1) K17776 - metaxin (MTX) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCTGTGACCTCCTGCCTGGCAGGCAGCGCGCCAGAGCCACGCCTGCTA |
| Internal bar code: | TTCGCCAGTAAGACGATGCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 356 |
| LEAP-Seq percent confirming: | 41.9355 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGGGAGAGGGGAAGAACT |
| Suggested primer 2: | GCGTTCCCGAATACACATGC |