| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.072420 |
| Chromosome: | plastome |
| Location: | 60300 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802288 | rps7,2716973,ChreCp024 | 30S ribosomal protein S7; (1 of 2) K02992 - small subunit ribosomal protein S7 (RP-S7, MRPS7, rpsG) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGGTTTACATACTCCGAAGTTTACTTGCCCGAAGGGGAAGGAGGAAGC |
| Internal bar code: | GAATTACTGCTATGCGAGGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 55 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTACCGCGCTTAAAGGCT |
| Suggested primer 2: | TTGCCATTGCTAACGCCATG |