Insertion junction: CLIP2.072434_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.072434
Chromosome:chromosome 1
Location:5515171
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g039250 RPS2 Cytosolic 80S ribosomal protein S2; (1 of 1) K02981 - small subunit ribosomal protein S2e (RP-S2e, RPS2) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGCTCGCTTTGGCCAGGCCATTATGCACTGGATTACAATCATGACATGG
Internal bar code:CAGGTAATCTGTATCTCATCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1112
LEAP-Seq percent confirming:87.5
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CTTACCGTATACCGCGTGCT
Suggested primer 2:TCATATTTGCGTGCGCCATG