| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.072439 |
| Chromosome: | chromosome 6 |
| Location: | 5734650 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g288450 | (1 of 1) PTHR14155//PTHR14155:SF102 - RING FINGER DOMAIN-CONTAINING // RING ZINC FINGER-CONTAINING PROTEIN | 3'UTR | |
| Cre06.g288500 | RRA2 | (1 of 3) PTHR10994//PTHR10994:SF3 - RETICULON // ARABINOSYLTRANSFERASE; Reduced residual arabinose 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCTATGCTAAGCTGCTGATGAGGTGGATGTTTGTGGATAGATGGCAT |
| Internal bar code: | ATAATTATTAGATTTTTGCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3356 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 110 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 110 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGGCATGTACTCGTGATA |
| Suggested primer 2: | CACGCCCCAATTTTTCCCAG |