| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.072447 |
| Chromosome: | chromosome 9 |
| Location: | 190272 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g800969 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGCTGAATGGAGAGCGACAAACACTCTTGTTATACTCAAGGGAGTGGG |
| Internal bar code: | TTAGAGGGATCTCGCGTGGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3726 |
| LEAP-Seq percent confirming: | 65.8228 |
| LEAP-Seq n confirming: | 52 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 79 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTCATGTTCGTTATGGGC |
| Suggested primer 2: | CAGACACGAACACGACCTGA |