| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.072450 |
| Chromosome: | chromosome 3 |
| Location: | 1895198 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g154850 | COQ4 | Ubiquinone biosynthesis protein; (1 of 1) K18586 - ubiquinone biosynthesis protein COQ4 (COQ4) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCGCCGCTGGCTGAGTCCCGACCTCACTCCGTGCGTCGTGTGCCTGC |
| Internal bar code: | TAACTACTCGGTTCGCTAGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1163 |
| LEAP-Seq percent confirming: | 92.0 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCGTTTGTGAATGCTGTT |
| Suggested primer 2: | CTCCAAGCCGAGGTTCCAAT |