Insertion junction: CLIP2.072452_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.072452
Chromosome:chromosome 6
Location:4915034
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g280900 VTI2,GOS1 (1 of 1) K08495 - golgi SNAP receptor complex member 1 (GOSR1, GOS1); Intra-Golgi Qb-SNARE, Gos1/Gos28-family (Qb.II) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGATCATATTTGTCTTTCTAACGTTTATTTGAACCCTACATCTTAGCAAC
Internal bar code:CAGATGGACCGGTTTGTAAGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:506
LEAP-Seq percent confirming:4.16667
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:23
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:GTATCCCCCTCACCTGGTCT
Suggested primer 2:GTGCTGGAAGTCGCTGATCT