| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.072458 |
| Chromosome: | chromosome 12 |
| Location: | 2544427 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g801380 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGTCTGCACTGTCTGCTGTCCTCTTGTGCGCGCACGCAGAGGGGCTAC |
| Internal bar code: | ACTTGGCTGGGAGGGTATGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3630 |
| LEAP-Seq percent confirming: | 4.54545 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCCTTTATGCTGCCGTGC |
| Suggested primer 2: | CTTACCCACAGAGCCACCAG |