Insertion junction: CLIP2.072469_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.072469
Chromosome:chromosome 6
Location:803584
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g255200 (1 of 31) IPR016187 - C-type lectin fold 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGCAAGTCCACATCAATTGACTCCACTGGACAAACTTTACCTTAGGCG
Internal bar code:TGCCGGGCTGTCGACATATAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1794
LEAP-Seq percent confirming:56.4516
LEAP-Seq n confirming:35
LEAP-Seq n nonconfirming:27
LEAP-Seq n unique pos:62

Suggested primers for confirmation by PCR

Suggested primer 1:GCGGGCTTGTTTGGATTTGT
Suggested primer 2:CGATACCCCTAGCGTGTTCC