Insertion junction: CLIP2.072486_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.072486
Chromosome:chromosome 6
Location:3638686
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278141 (1 of 58) PTHR12393//PTHR12393:SF6 - SPHINGOMYELIN PHOSPHODIESTERASE RELATED // SPHINGOMYELIN PHOSPHODIESTERASE 2 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTGCGTAGCCGCACGCACTCGCAGCCACAGCCGGCCGCAACAGCCGCAG
Internal bar code:ATTAGTCTTGTGGTGTCCTGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2106
LEAP-Seq percent confirming:69.7674
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:43

Suggested primers for confirmation by PCR

Suggested primer 1:CTCGTCGGCTATGTCAGGTC
Suggested primer 2:GGGCCTGAAAATTGCCGTTT