Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.072495 |
Chromosome: | plastome |
Location: | 129434 |
Confidence (%): | 63 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
CreCp.g802318 | ChreCp054,2716983,atpF | ATP synthase CF0 B subunit; (1 of 1) PTHR34264:SF3 - ATP SYNTHASE SUBUNIT B, CHLOROPLASTIC | 5'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACTGAAATTTAAGCTAAGTCAAATTTATTTTCTCACTTTCAAGGCTGCG |
Internal bar code: | TGCATCATCATCTTAGCACGCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 614 |
LEAP-Seq percent confirming: | 54.5455 |
LEAP-Seq n confirming: | 18 |
LEAP-Seq n nonconfirming: | 15 |
LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACTGCCTTTGGTGCTTCCTT |
Suggested primer 2: | AGTAGTACCGCCACTGCCTA |