Insertion junction: CLIP2.072499_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.072499
Chromosome:chromosome 16
Location:7329031
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre16.g678997 (1 of 2) 2.4.2.38 - Glycoprotein 2-beta-D-xylosyltransferase / Beta-1,2-xylosyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGACGTGTGGGCGTCGCTGTCGCGGCCCTACCCGCTGCGCATCCTGTCC
Internal bar code:TCAGCCGACAGTCGTGGAACTC

Confirmation - LEAP-Seq

LEAP-Seq distance:198
LEAP-Seq percent confirming:10.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:TCCAAACATGCGGGTATGCT
Suggested primer 2:TGGTCACCACCTTCATCAGC