| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.072514 |
| Chromosome: | chromosome 3 |
| Location: | 8938909 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g204465 | GT90F36,GT90-36 | GT90 family protein 36; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) | 5'UTR |
| Cre03.g204577 | DNJ31 | (1 of 1) IPR000104//IPR001623//IPR003072 - Antifreeze protein, type I // DnaJ domain // Orphan nuclear receptor, NOR1 type; DnaJ-like protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGCCTCCACGGTTTCCTGCGAAACTTGAGATATCTCTAGTCTTTGACT |
| Internal bar code: | AACTTTGACTTTAATTTAATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4324 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTTTCGCTTGTGCAGTTG |
| Suggested primer 2: | GACAGGTCCCTCTGGATCCT |