| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.072515 |
| Chromosome: | chromosome 1 |
| Location: | 848136 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g004750 | (1 of 3) IPR000104//IPR002048 - Antifreeze protein, type I // EF-hand domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAAGCCCTGTTATGCGAGCGCGTGCGTGAAGGCGAACGTCAATTGGGGG |
| Internal bar code: | TCTTTATAGGCGGATGTGATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 128 |
| LEAP-Seq percent confirming: | 8.33333 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGAGCTGACATCATAGCGT |
| Suggested primer 2: | GTAGGAGCAGGCTCGTGTTT |