Insertion junction: CLIP2.072517_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.072517
Chromosome:chromosome 12
Location:9005361
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre12.g545101 XDH1 (1 of 1) 1.17.1.4 - Xanthine dehydrogenase / Xanthine/NAD(+) oxidoreductase; Xanthine dehydrogenase/oxidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGGCGTGCCCGCACACAAGATCGTGTCCAAGGTACGTGTACACACACA
Internal bar code:ACAGAGGGGCTGGATCGCCAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:173
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTGTGTGTGCATGGTGTG
Suggested primer 2:GAGGGAGGTGCAAGAACGAA