Insertion junction: CLIP2.072523_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.072523
Chromosome:chromosome 3
Location:4505331
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g175851 (1 of 13) IPR001487 - Bromodomain 5'UTR_intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAACCCCTCCTCTCTCCATCCATCACGACAGTATCCACCGGCGCCGTGG
Internal bar code:GTCATAGGCAATGGGGAACCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:4431
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GTTATCTCGGCGATGTGGGT
Suggested primer 2:AAGAGGCTTGCTGCTAGGTG