| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.072528 |
| Chromosome: | chromosome 9 |
| Location: | 3598019 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396772 | Similar to Gametolysin and Autolysin; (1 of 4) IPR003882//IPR008752 - Pistil-specific extensin-like protein // Peptidase M11, gametolysin | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCCAGAGGAAATGGCTGCTGCCTGTCCCCTTCTGCGCGCACCACGCAA |
| Internal bar code: | ATGCATAGGTTTCCTAGATATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1008 |
| LEAP-Seq percent confirming: | 95.5882 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTGACCGTGACCAGAAAG |
| Suggested primer 2: | CACTGCTGGAGCCAGAAAGA |