| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.072529 |
| Chromosome: | chromosome 13 |
| Location: | 985369 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g568350 | BLZ18 | bZIP transcription factor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATACACATGCACACAAGCACAAGTCCCCTGACCCCCCCCCCCCCCGCA |
| Internal bar code: | TACTGGGTTAATTACACCTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2710 |
| LEAP-Seq percent confirming: | 96.4286 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCACGCTTACTTCATTCCG |
| Suggested primer 2: | CAGGGTGATACAGCAAGCCA |