| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.072538 |
| Chromosome: | chromosome 3 |
| Location: | 8662781 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g209617 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATCAATGCTTAACAAATAGGACAATAAGATAATAGTATCAATTGTCAG |
| Internal bar code: | ACTGCTGTCAAGCACTACGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 844 |
| LEAP-Seq percent confirming: | 76.3158 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTGATAGGTCCGTGCA |
| Suggested primer 2: | GATACCGGGTGCAGGATGAG |