| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.072548 |
| Chromosome: | chromosome 7 |
| Location: | 4758256 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g800840 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGGGCGACTACGTGTGCGCGGCGACGCGCGACTTCTACTCGGCGCTGG |
| Internal bar code: | CCGGTAGAAAAACAGCAGAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1005 |
| LEAP-Seq percent confirming: | 4.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTCGTCTAAGCGGTCAC |
| Suggested primer 2: | CACAATCCACCTCTCCTCCG |