| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.072566 |
| Chromosome: | chromosome 1 |
| Location: | 3685150 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g023850 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGTGTGCAGTCTTTGCTGGCGAGCGTAGCGTGGCACGGTTGCTGCAAC |
| Internal bar code: | GTTTTCAAAGCGGAGGTCGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2048 |
| LEAP-Seq percent confirming: | 62.5 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTTGAGGTGGGTATGGGG |
| Suggested primer 2: | CACAACACAATGCGCTCACA |