Insertion junction: CLIP2.072570_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.072570
Chromosome:chromosome 9
Location:2909505
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre09.g390450 POB15 Proteome of basal body 15; (1 of 88) IPR011333 - POZ domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTCGTGCCACACACAGCCACACATTACAGTATTCATTGATTGTCATGCT
Internal bar code:CAACGCGCGCCTCATAAGGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:270
LEAP-Seq percent confirming:24.1379
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:66
LEAP-Seq n unique pos:87

Suggested primers for confirmation by PCR

Suggested primer 1:AGGGAGCAAACACACACACA
Suggested primer 2:AAGGCGGTACTGATGTCGTG