| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.072574 |
| Chromosome: | chromosome 6 |
| Location: | 8757176 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g310800 | (1 of 781) IPR000104 - Antifreeze protein, type I | 5'UTR | |
| Cre06.g310850 | (1 of 12) IPR003439//IPR003593//IPR027417 - ABC transporter-like // AAA+ ATPase domain // P-loop containing nucleoside triphosphate hydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAAACGCCCGACCTTTTGCCCCGAGCAGTAAAAATGGTGATTATATTAA |
| Internal bar code: | CTGCTAGTGGTCCTGAGTGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 144 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGCACTAGCTACTGCCGC |
| Suggested primer 2: | CGCGGCGTATGAGTAAGACT |