| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.072577 |
| Chromosome: | chromosome 16 |
| Location: | 4541165 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g671400 | ARS1 | (1 of 2) IPR000917//IPR012083//IPR017850 - Sulfatase // Arylsulfatase // Alkaline-phosphatase-like, core domain; Periplasmic arylsulfatase 1 | 5'UTR |
| Cre16.g671450 | FAO12 | FAD-dependent oxidoreductase; (1 of 1) PTHR13847//PTHR13847:SF150 - FAD NAD BINDING OXIDOREDUCTASES // OXIDOREDUCTASE TDA3-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGCAGCGGCCATTCTTAGCATCGCGCGTTTTCATATGCGCTCTACGTA |
| Internal bar code: | CTAAGGGGGTGCGTGTGGGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2349 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTATCTGCCCTGAGAGCA |
| Suggested primer 2: | CGCTTGCAACTCGACCAAAA |