| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.072579 |
| Chromosome: | plastome |
| Location: | 15024 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802268 | 2717012,rpl20,ChreCp006 | 50S ribosomal protein L20; (1 of 1) PTHR10986//PTHR10986:SF12 - 39S RIBOSOMAL PROTEIN L20 // 50S RIBOSOMAL PROTEIN L20, CHLOROPLASTIC | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTAACTTGTTTATCTAGTAAATAAATTGGAGTTCTTCAAAGCAGAGTC |
| Internal bar code: | TCAGGTTGTGTACGTGGTGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 113 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGGCCCAGTAGGAATCGA |
| Suggested primer 2: | TGAAAATTGGCGTGGCTTCG |