| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.072581 |
| Chromosome: | chromosome 5 |
| Location: | 1882869 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g235800 | FBB19 | Flagellar/basal body protein 19 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGACCGCCGCTGCTACCATCCAACACCCCTCACCGATTTCCTCCACA |
| Internal bar code: | GTGGGCACCTCTCGTGTTACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 72 |
| LEAP-Seq percent confirming: | 17.3913 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGGAGTTGAAGGACTTGA |
| Suggested primer 2: | AATCCTTTGCGCCTACTGCT |