| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.072596 |
| Chromosome: | chromosome 10 |
| Location: | 1239533 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g426450 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAGGAACGTCGAACTTGAAGCGCGCGTGGCACAGGCGTGGCAGATACGC |
| Internal bar code: | TTAGACACTGCTGGTCTAGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 287 |
| LEAP-Seq percent confirming: | 86.0465 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGTGTGCTGCTGGTTCAG |
| Suggested primer 2: | GCCGTGATAGCTCTGCTAGG |