| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.072614 |
| Chromosome: | chromosome 2 |
| Location: | 2391466 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g091050 | ALAD1,PBGS,HEM2,ALAD | (1 of 1) 4.2.1.24 - Porphobilinogen synthase / Delta-aminolevulinic acid dehydratase; Delta-aminolevulinic acid dehydratase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACAATAGGAAGGAGCGACGCGCCCGCAGGGGGTAGTTGGGGGCAACAG |
| Internal bar code: | CTTTTTGCTGCGCTCCGAGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 397 |
| LEAP-Seq percent confirming: | 24.1379 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTGCAGATGGGAGTGACG |
| Suggested primer 2: | AGGAGAGCAACCAGAACGTG |