| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.072617 |
| Chromosome: | chromosome 12 |
| Location: | 2608894 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g506350 | HFO18 | (1 of 33) K11254 - histone H4 (H4); Histone H4 | 3'UTR |
| Cre12.g506400 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGCGCCCCTCCACCCTCCACTGTAAGTGCATGTCAACACCCAACCCG |
| Internal bar code: | GTACTTTGGGTCGGGTAGAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2186 |
| LEAP-Seq percent confirming: | 71.4286 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAATCGTGGGCGGTTGTTTG |
| Suggested primer 2: | CTGGGTTTTGGACTGGGTGT |